Asahi Glass Company mitmekesistamise strateegia juhtumi analuus

Ideas, Policies and Challenges. Jaapani majanduse kõigis valdkondades on olemas kvaliteediringid, kuhu lisaks töötajatele kuuluvad ka käsitöölised ja insenerid. Ja lõpuks püüdsid nad alati kvaliteeti ja usaldusväärsust parandada võimalikult kõrgel ja seejärel kõrgemal tasemel; tasemeni, mida konkurendid lihtsalt ei suuda saavutada. Kõik muudatused võivad põhjustada vooluhäireid, mis võivad laieneda kogu tarneahelas, põhjustades katkestusi ja viivitusi. Vist esimest korda pärast iseseisvuse taastamist muutub Eesti aeglasemalt kui meid ümbritsev maailm.

Asahi Glass Company mitmekesistamise strateegia juhtumi analuus

Villarreal, Seth A. Here, we present a crystallographic structure of the wild-type Synechococcus elongatus KaiB and a cryo-electron microscopy cryoEM structure of a KaiBC complex. The crystal structure shows the expected dimer core structure and significant conformational variations of the KaiB C-terminal region, which is functionally important in maintaining rhythmicity.

Asahi Glass Company mitmekesistamise strateegia juhtumi analuus

A KaiC mutation, RC, which has been shown to affect the affinity of KaiB for KaiC and lengthen the period in a bioluminescence rhythm assay, is found within the middle of the predicted KaiBC interface. We demonstrate here that the AV KaiC mutant sheds light on the former mechanism.

Asahi Glass Company mitmekesistamise strateegia juhtumi analuus

It was previously reported that AV is less sensitive to dark pulse-induced phase resetting and has a reduced amplitude of the KaiC phosphorylation rhythm in vivo. A maps to a loop loop that continues toward the phosphorylation sites.

Asahi Glass Company mitmekesistamise strateegia juhtumi analuus

By pulling on the C-terminal peptide of KaiC A-loopKaiA removes restraints from the adjacent loop whose increased flexibility indirectly promotes kinase activity. We found in the crystal structure that AV KaiC lacks phosphorylation at S and exhibits a subtle, local conformational change relative to wild-type KaiC.

Asahi Glass Company mitmekesistamise strateegia juhtumi analuus

Molecular dynamics simulations indicate higher mobility of the loop in the absence of the A-loop and mobility differences in other areas associated with phosphorylation activity between wild-type and mutant KaiCs. Finally, the A-loop—loop relay that informs KaiC phosphorylation sites of KaiA dimer binding propagates to loops from neighboring KaiC subunits, thus providing support for a concerted allosteric mechanism of phosphorylation.